Archeologist and avid puzzle solver Professor Layton and his assistant Luke are caught up in an adventure when a masked figure steals an entire opera house and forces those in attendance to play a high-stakes game. The winner will receive eternal life, but it could mean death for the losers.

Hikaru is given a phone call asking him to appear in the Japan/China/Korea Junior's Tournament but he finds out he will have to take part in a preliminary tournament to choose Japan's three contestants. Upon discussing this with Akira, he discovers that Akira has already been chosen for the tournament and will not be taking place in the preliminaries. Hikaru then decides not to visit Akira's Go Salon until he takes his place on the team with Akira. That may be tougher than originally planned...

In Okinawa, Ayaka Kurenai was born in the world’s greatest legendary Karate family. When she was a child, her family was attacked by a mysterious gang that seeks legendary black belt. Her father Soujiro Kurenai was killed and her younger sister was taken in front of her. Time has passed. In Yokohama, she was spending her life as a normal high school girl, hiding her real name and her status as a heir of the legendary Karate family. However, one incident brought her a fateful encounter with her sister who was separated in their childhood. But, her sister was raised as a heartless killing machine by the mysterious gang who took her. .