Four survivors find themselves stranded aboard a small escape pod in deep space. Trying to piece together the details around an outbreak, they find themselves unsure to trust whether or not one of them might be infected.

During the night shift in a colony greenhouse, a botanist does her best to contain suspicious soil samples that have alarmed her sensitive lab dog.

When a missing space trucker is discovered hungover and disoriented, his co-worker suggests a nightcap as a remedy. Near closing time, they are reluctantly allowed inside the colony supply depot where the trucker’s condition worsens, leaving a young supply worker alone to take matters into her own hands. Alien: Night Shift is one of six short films produced to celebrate the 40th anniversary of 1979's Alien.

The surviving crew of a damaged space harvester has a motion sensor as their only navigation tool leading them to safety, while a creature in the shadows terrorizes them. However, the greatest threat might have been hiding in plain sight.

On the night of an astronomical anomaly, eight friends at a dinner party experience a troubling chain of reality bending events.